Multiple sclerosis-like syndrome work-up

 

Screening tests

Serum glucose, electrolytes, calcium/phosphorus, uric acid, lactate and pyruvate, liver, renal and thyroid function tests, blood gasses, osmolality

Serum CK

ANA, ENA, ANCA, RF, complement, SSA- and SSB autoantibodies, ACE

CBC, ESR,

Serum vitamin B12 and folic acid

RPR, TPHA

Serology: HIV, HSV, adenovirus, CMV, Coxsackie, polio, echovirus, hepatitis (A,B,C), parvovirus B19, brucella, C. jejuni, Lyme, legionella, chlamydia,  mycoplasma, toxoplasma, VDRL, cysticercosis

Chest X-ray

PPD

Echocardiogram

EEG (non-convulsive status epilepticus), VEP, SEP, BAEP, EMG/NCVs

Brain MRI w/o and w/ gadolinium, MRA

CSF

Besides routine analysis (chemistry, cell count, smear and stainings): lactate and pyruvate (mitochondrial disease), oligoclonal bands, IgG index, VDRL, viral (measles titer), fungal, PCR (TB, T. Whippleii, JC virus, HSV, HHV6, EBV, CMV, VZV), Ziehl staining, repeated cytology,

anti-thyroglobulin and anti-thyroperoxidase antibodies (Hashimoto disease).

 

 

Specific investigations

Blood/serum

Blood (and urine) organic acids and carnitine

Anti-thyroglobulin and anti-thyroperoxidase antibodies (Hashimoto disease), immunoglobulin electrophoresis

Coagulation profile (protein C and S, ATIII, Factor Leiden V, APL antibodies, anticardiolipin antibodies)

Anti-Hu, anti-Yo antibodies

Methylmalonic acid, VLCFA, arylsulphatase, homocysteine

Miscellaneous

Conventional angiogram (CNS vasculitis)

Genetic testing

 

Trinucleotide repeats for SCA 1,2,3,6,7,10,12, DRPLA, Friedrich ataxia (frataxin) and HSP (spastin)

 

Mitochondrial mutation analysis

 

 

Biopsy

 

Conjunctiva (sarcoidosis),

Small bowel (Whipple disease)

Skin (SLE, vasculitis, CADASIL)

Brain biopsy

 

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